Coding

Part:BBa_K3788015:Design

Designed by: Rebecca Pags   Group: iGEM21_Aix-Marseille   (2021-09-28)


Modified lysis device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 585
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 905
    Illegal BamHI site found at 578
    Illegal XhoI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 408


Design Notes

Source

References

- Cascales, E. et al. Colicin biology. Microbiol. Mol. Biol. Rev. MMBR71, 158–229 (2007).

- Cavard, D. Role of Cal, the colicin A lysis protein, in two steps of colicin A release and in the interaction with colicin A-porin complexes. Microbiol. Read. Engl. 150, 3867–3875 (2004).

- Lloubes, R., Baly, D. & Lazdunski, C. The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and Lex A protein. Nucleic Acids Res . 14, 2621–2636 (1986).

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